Crown gall tumors: are bacterial nucleic acids involved?
نویسندگان
چکیده
منابع مشابه
Revertant seedlings from crown gall tumors retain a portion of the bacterial Ti plasmid DNA sequences.
BT37 is a crown gall teratoma incited on tobacco by Agrobacterium tumefaciens containing pTi-T37, a nopaline-type Ti plasmid. Treatment of this cloned tumor tissue with kinetin at 1 mg/liter results in the formation of relatively normal-appearing shoots. These shoots can be induced to root and set viable seed. In contrast to BT37 tissue, the derived tissues are not phytohormone independent and ...
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Crown gall tumors collected from naturally infected plants of 7 grapevine varieties were analysed for the presence of opines. Eighty-five of the tested 90 samples contained known Agrobacterium vitis-induced opines. Octopine was the most common, it was found in 50 samples. Twentyeight crown galls contained nopaline and 8 were vitopine positive. There was only one tumor that contained two opines,...
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A procedure is described that permits the use of radiotracer methods for examining crown gall tumors in the presence of the initiating agent Agrobacterium tumefaciens. On the basis of sensitivity testing using the disc diffusion technique and the minimum inhibitory concentration procedure and incorporation of radioactive phosphorus into nucleic acids, it was determined that A. tumefaciens was m...
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DNA is the most typical nucleic acid that is sequenced. Researcher will isolate total DNA, create some sequencing library, and those libraries will be sequenced using the Sanger approach or any of the modern next generation sequence technologies. But that is not the only type of nucleic acid that is sequenced. Often a researcher would like to study just a fraction of the genome rather than all ...
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ژورنال
عنوان ژورنال: Bacteriological Reviews
سال: 1975
ISSN: 0005-3678
DOI: 10.1128/br.39.3.186-196.1975